polyclonal antihuman gh antibody  (R&D Systems)

Journal: Journal of Virology

Article Title: Kaposi’s sarcoma-associated herpesvirus glycoprotein K8.1 is critical for infection in a cell-specific manner and functions at the attachment step on keratinocytes

doi: 10.1128/jvi.00832-23

Figure Lengend Snippet: KSHVΔK8.1 exhibits reduced attachment on HaCaT cells. (A) SLK, HaCaT, A549, or 293T cells were spin infected at 4°C with the indicated amounts of virus, and bound viral DNA was quantified by qPCR. Binding curves for KSHV wt and KSHVΔK8.1 were indistinguishable on SLK but significantly different for HaCaT and A549 (non-linear fit for semi-log data by least squares regression using mean values for each data point, slope shared between curves, ratio calculated from Y-intercept; two curves and * indicate significant difference with P < 0.05; n = 6). (B) Binding of Fc-fusion proteins K8.1-Fc, KSHV gH-Fc/gL, R8.1-Fc, or RRV gHΔ21–27-Fc (control/background) to the indicated cells was measured by flow cytometry. Error bars represent the standard deviation, n = 3.

Article Snippet: K8.1 was detected using monoclonal antibody BS555 ( 60 ), a kind gift from Frank Neipel. gH was detected using custom-made polyclonal rabbit antibodies raised against peptides derived from KSHV gH (Genscript).

Techniques: Infection, Virus, Binding Assay, Control, Flow Cytometry, Standard Deviation

Journal: Journal of Virology

Article Title: Kaposi’s sarcoma-associated herpesvirus glycoprotein K8.1 is critical for infection in a cell-specific manner and functions at the attachment step on keratinocytes

doi: 10.1128/jvi.00832-23

Figure Lengend Snippet: Presence or absence of K8.1 or its RRV homolog does not appreciably modulate gH/gL-activated membrane fusion by gB. (A) KSHV gH/gL together with RRV gB, or KSHV gH together with RRV gB, was expressed in the presence or absence of K8.1 on 293T effector cells, and these cells were cocultured with the indicated target cell populations. Error bars represent the standard deviation of triplicate samples. (B) RRV gH/gL together with RRV gB, or RRV gH together with RRV gB, was expressed in the presence or absence of R8.1 on 293T effector cells, and these cells were cocultured with the indicated target cell populations. Error bars represent the standard deviation of triplicate samples.

Article Snippet: K8.1 was detected using monoclonal antibody BS555 ( 60 ), a kind gift from Frank Neipel. gH was detected using custom-made polyclonal rabbit antibodies raised against peptides derived from KSHV gH (Genscript).

Techniques: Membrane, Standard Deviation